rpsU

168

ribosomal protein bS21

Locus: BSU_25410

Molecular weight: 6.69 kDa

Isoelectric point: 11.58

Protein length: 57 aa

Gene length: 174 bp

Function: translation

Product: ribosomal protein S21 (bS21)

Essential: no

Synonyms: rpsU, yqeX

Genomic Context

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List of homologs in different organisms, belongs to COG0828 (Galperin et al., 2021)

Gene

Coordinates: 2,620,371  2,620,544

The protein

Protein family

• bacterial ribosomal protein bS21 family (single member, according to UniProt)

Structure

• 3J9W (PDB) (the ribosome) PubMed
• P21478 (AlphaFold)

Additional information

• this ribosomal protein is lacking in some bacteria PubMed

Expression and Regulation

Operons

• Genes: rpsU-yqeY

  Description: PubMed

  Regulation

  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Expression

  • expression is increased upon depletion of depletion of tRNA maturation factors rnpB or rnz PubMed

Biological materials

Mutant

• BKE25410 (rpsU::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCTTTCCCTCCCTCC,  downstream forward: _UP4_AAATTCTAAAAGAGGGTGGA
• BKK25410 (rpsU::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCTTTCCCTCCCTCC,  downstream forward: _UP4_AAATTCTAAAAGAGGGTGGA

References

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