QueE

• Description: 7-carboxy-7-deazaguanine (CDG) synthase, required for the synthesis of the modified ribonucleotide queuosine
  
  

Gene name	queE
Synonyms	ykvL
Essential	no
Product	7-carboxy-7-deazaguanine (CDG) synthase
Function	tRNA modification
Gene expression levels in SubtiExpress: queE
Interactions involving this protein in SubtInteract: QueE
MW, pI	26 kDa, 4.962
Gene length, protein length	729 bp, 243 aa
Immediate neighbours	queD, queF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

preQ1 riboswitch

The gene

Basic information

• Locus tag: BSU13740

Database entries

• BsubCyc: BSU13740

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• • conversion of 6-carboxy-5,6,7,8-tetrahydropterin to 7-carboxy-7-deazaguanine (CDG) PubMed

• Catalyzed reaction/ biological activity:

• Protein family: radical S-adenosyl-L-methionine (SAM) superfamily

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): [4Fe-4S] cluster, S-adenosyl-L-methionine, Mg(2+) PubMed

• Effectors of protein activity:

• Interactions:
  • QueE-YkuN (electron transfer) PubMed

• Localization:

Database entries

• BsubCyc: BSU13740

• Structure:

• UniProt: O31677

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: queC-queD-queE-queF PubMed

• Expression browser: queE PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of queuosine (preQ1 riboswitch) PubMed

• Regulatory mechanism:
  • preQ1 riboswitch: transcriptional antitermination in the absence of queuosine PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Nathan A Bruender, Anthony P Young, Vahe Bandarian
Chemical and Biological Reduction of the Radical SAM Enzyme 7-Carboxy-7-deazaguanine [corrected] Synthase.
Biochemistry: 2015, 54(18);2903-10
[PubMed:25933252] [WorldCat.org] [DOI] (I p)

Daniel P Dowling, Nathan A Bruender, Anthony P Young, Reid M McCarty, Vahe Bandarian, Catherine L Drennan
Radical SAM enzyme QueE defines a new minimal core fold and metal-dependent mechanism.
Nat Chem Biol: 2014, 10(2);106-12
[PubMed:24362703] [WorldCat.org] [DOI] (I p)

Reid M McCarty, Carsten Krebs, Vahe Bandarian
Spectroscopic, steady-state kinetic, and mechanistic characterization of the radical SAM enzyme QueE, which catalyzes a complex cyclization reaction in the biosynthesis of 7-deazapurines.
Biochemistry: 2013, 52(1);188-98
[PubMed:23194065] [WorldCat.org] [DOI] (I p)

Reid M McCarty, Arpád Somogyi, Guangxin Lin, Neil E Jacobsen, Vahe Bandarian
The deazapurine biosynthetic pathway revealed: in vitro enzymatic synthesis of PreQ(0) from guanosine 5'-triphosphate in four steps.
Biochemistry: 2009, 48(18);3847-52
[PubMed:19354300] [WorldCat.org] [DOI] (I p)

Mijeong Kang, Robert Peterson, Juli Feigon
Structural Insights into riboswitch control of the biosynthesis of queuosine, a modified nucleotide found in the anticodon of tRNA.
Mol Cell: 2009, 33(6);784-90
[PubMed:19285444] [WorldCat.org] [DOI] (I p)

Adam Roth, Wade C Winkler, Elizabeth E Regulski, Bobby W K Lee, Jinsoo Lim, Inbal Jona, Jeffrey E Barrick, Ankita Ritwik, Jane N Kim, Rüdiger Welz, Dirk Iwata-Reuyl, Ronald R Breaker
A riboswitch selective for the queuosine precursor preQ1 contains an unusually small aptamer domain.
Nat Struct Mol Biol: 2007, 14(4);308-17
[PubMed:17384645] [WorldCat.org] [DOI] (P p)

John S Reader, David Metzgar, Paul Schimmel, Valérie de Crécy-Lagard
Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine.
J Biol Chem: 2004, 279(8);6280-5
[PubMed:14660578] [WorldCat.org] [DOI] (P p)