cwlK

168

peptidoglycan hydrolytic L,D-endopeptidase

Locus: BSU_02810

Molecular weight: 18.97 kDa

Isoelectric point: 5.66

Protein length: 167 aa

Gene length: 504 bp

Function: cell wall turnover

Product: peptidoglycan hydrolytic L,D-endopeptidase

Essential: no

Synonyms: cwlK, ycdD

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 303,804 → 304,307

The protein

Catalyzed reaction/ biological activity

• cleaves the peptide bond between L-Ala (position 1 in the peptioglycan peptide) and D-Glu (position 2) PubMed

Protein family

• peptidase M15C family (single member, according to UniProt)

Structure

• 6AKV (PDB) (from Bacillus phage B4, correpsonds to aa 61 ... 164, 51% identity) PubMed
• O34360 (AlphaFold)

Localization

• cell membrane PubMed

Biological materials

Mutant

• BKE02810 (ΔcwlK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCGGTCTCACTTTCTC,  downstream forward: _UP4_GAGATGATTCCTAACTAGAC
• BKK02810 (ΔcwlK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCGGTCTCACTTTCTC,  downstream forward: _UP4_GAGATGATTCCTAACTAGAC

Labs working on this gene/protein

• Ciaran Condon, IBPC, Paris, France Homepage

References

Reviews

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Original Publications

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