nrdEB

168

similar to ribonucleoside-diphosphate reductase (alpha subunit)

Locus: BSU_20060

Molecular weight: 27.82 kDa

Isoelectric point: 6.39

Protein length: 1168 aa

Gene length: 3507 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: nrdEB, yosN

Genomic Context

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Categories containing this gene/protein

Metabolism, Nucleotide metabolism, Biosynthesis/ acquisition of nucleotides, Biosynthesis/ acquisition of nucleotides/ other

Prophages and mobile genetic elements, Prophages, SP-beta prophage

List of homologs in different organisms, belongs to COG1372 (Galperin et al., 2021)

Gene

Coordinates: 2,162,108 → 2,165,614

The protein

Catalyzed reaction/ biological activity

[thioredoxin]-disulfide + 2'-deoxyribonucleoside 5'-diphosphate + H₂O --> [thioredoxin]-dithiol + ribonucleoside 5'-diphosphate (according to UniProt)

Protein family

ribonucleoside diphosphate reductase large chain family (with NrdE, according to UniProt)

Domains

DOD-type homing endonuclease domain (aa 503-654) (according to UniProt)

Structure

O31875 (AlphaFold)

Paralogous protein(s)

NrdE

Biological materials

Mutant

BKE20060 (ΔnrdEB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTGAGCTTGATCCAA,  downstream forward: _UP4_TAATAGGTACGTTTACGTTT
BKK20060 (ΔnrdEB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTGAGCTTGATCCAA,  downstream forward: _UP4_TAATAGGTACGTTTACGTTT

References

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