nrdEB

168

similar to ribonucleoside-diphosphate reductase (alpha subunit)

locus: BSU_20060

Molecular weight: 27.82 kDa

pI: 6.39

Protein length: 1168 aa

Gene length: 3507 bp

function: unknown

product: unknown

essential: no

synonyms: nrdEB, yosN

List of homologs in different organisms, belongs to COG1372 (Galperin et al., 2021)

Gene

Coordinates: 2,162,108 → 2,165,614

The protein

Catalyzed reaction/ biological activity

• [thioredoxin]-disulfide + 2'-deoxyribonucleoside 5'-diphosphate + H₂O --> [thioredoxin]-dithiol + ribonucleoside 5'-diphosphate (according to UniProt)

Protein family

• ribonucleoside diphosphate reductase large chain family (with [protein|3B68CE8E9A35997B45E437A316996D585E43B76B|nrdE], according to UniProt)

[wiki|Domains]

• DOD-type homing endonuclease domain (aa 503-654) (according to UniProt)

Structure

• [AF|O31875]

Paralogous protein(s)

• [protein|3B68CE8E9A35997B45E437A316996D585E43B76B|nrdE]

Biological materials

Mutant

• BKE20060 (Δ[gene|FD4CD7DB35AA20DBBEC0A4DA5D88282D89CCDA6D|nrdEB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE20060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTGAGCTTGATCCAA,  downstream forward: _UP4_TAATAGGTACGTTTACGTTT
• BKK20060 (Δ[gene|FD4CD7DB35AA20DBBEC0A4DA5D88282D89CCDA6D|nrdEB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK20060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTGAGCTTGATCCAA,  downstream forward: _UP4_TAATAGGTACGTTTACGTTT

References

• 21498918,11470879,16621400,9465078