ssuC

168

aliphatic sulfonate ABC transporter (permease)

Locus: BSU_08850

Molecular weight: 30.09 kDa

Isoelectric point: 10.48

Protein length: 276 aa

Gene length: 831 bp

Function: sulfonate uptake

Product: aliphatic sulfonate ABC transporter (permease)

Essential: no

Synonyms: ssuC, ygaM, yzeB

Genomic Context

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List of homologs in different organisms, belongs to COG0600 (Galperin et al., 2021)

Gene

Coordinates: 963,174  964,004

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• CysTW subfamily (according to UniProt)

Domains

• ABC transmembrane type-1 domain(aa 80-260) (according to UniProt)

Structure

• P40401 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: ssuB-ssuA-ssuC-ssuD-ygaN

  Description: PubMed

  Regulation

  • repressed in the presence of cysteine (CymR) PubMed

  Regulatory mechanism

  • CymR: repression, PubMed, in cymR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP4466 (trpC2 ΔssuC::neo), available in Jörg Stülke's lab
• MGNA-A247 (ygaM::erm), available at the NBRP B. subtilis, Japan
• 1A800 ( ssuC::kan), PubMed, available at BGSC
• BKE08850 (ssuC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGAGCCCGCAGCCTCTGCTT,  downstream forward: _UP4_TAAAAGATATAAAGGGGAGA
• BKK08850 (ssuC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGAGCCCGCAGCCTCTGCTT,  downstream forward: _UP4_TAAAAGATATAAAGGGGAGA

References

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