secE

168

preprotein translocase subunit

Locus: BSU_01000

Molecular weight: 6.79 kDa

Isoelectric point: 10.47

Protein length: 59 aa

Gene length: 180 bp

Function: protein secretion

Product: preprotein translocase subunit

Essential: yes

Synonyms: secE

Genomic Context

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Categories containing this gene/protein

Information processing, Protein synthesis, modification and degradation, Protein secretion

Groups of genes, Essential genes

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG0690 (Galperin et al., 2021)

Gene

Coordinates: 117,532  117,711

Phenotypes of a mutant

essential PubMed PubMed

The protein

Protein family

SecE/SEC61-gamma family (single member, according to UniProt)

Domains

transmembrane protein with a single TM domain

Structure

3DL8 (PDB) (structure of the (SecY-SecE-SecG)-SecA complex) PubMed
Q06799 (AlphaFold)

Localization

cell membrane (according to UniProt)

Additional information

B. subtilis secE complements the secretion phenotype of a cold sensitive E. coli secE mutant.

Expression and Regulation

Operons

Genes: sigH-rpmGB-secE

Description: PubMed

Regulation

repressed during logrithmic growth (AbrB) PubMed

Regulatory mechanism

AbrB: repression, PubMed, in abrB regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

References

Reviews

Tsirigotaki A, De Geyter J, Šoštaric N, Economou A, Karamanou S Protein export through the bacterial Sec pathway. Nature reviews. Microbiology. 2017 Jan; 15(1):21-36. doi:10.1038/nrmicro.2016.161. PMID:27890920
Kang Z, Yang S, Du G, Chen J Molecular engineering of secretory machinery components for high-level secretion of proteins in Bacillus species. Journal of industrial microbiology & biotechnology. 2014 Nov; 41(11):1599-607. doi:10.1007/s10295-014-1506-4. PMID:25212246

Original Publications

Zheng Z, Blum A, Banerjee T, Wang Q, Dantis V, Oliver D Determination of the Oligomeric State of SecYEG Protein Secretion Channel Complex Using in Vivo Photo- and Disulfide Cross-linking. The Journal of biological chemistry. 2016 Mar 11; 291(11):5997-6010. doi:10.1074/jbc.M115.694844. PMID:26747607
Zimmer J, Nam Y, Rapoport TA Structure of a complex of the ATPase SecA and the protein-translocation channel. Nature. 2008 Oct 16; 455(7215):936-43. doi:10.1038/nature07335. PMID:18923516
Jeong SM, Yoshikawa H, Takahashi H Isolation and characterization of the secE homologue gene of Bacillus subtilis. Molecular microbiology. 1993 Oct; 10(1):133-42. . PMID:7968510