yugI

168

similar to the C-terminal domain of E. coli polyribonucleotide phosphorylase and to four repeated domains at the N-terminus of E. coli ribosomal protein S1, has an RNA-binding surface

Locus: BSU_31390

Molecular weight: 14.14 kDa

Isoelectric point: 5.92

Protein length: 130 aa

Gene length: 393 bp

Function: unknown

Product: GSP13

Essential: no

Synonyms: yugI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1098 (Galperin et al., 2021)

Gene

Coordinates: 3,225,178 3,225,570

The protein

Domains

• S1 domain (aa 8-77) (according to UniProt)

Structure

• 2K4K (PDB) PubMed
• P80870 (AlphaFold)

Paralogous protein(s)

• YabR, Array

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: yugI

  Description: PubMed

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Additional information

  • A ncRNA is predicted between 'yugI' and 'alaT' PubMed

Biological materials

Mutant

• GP3597 (ΔyugI::cat), available in Jörg Stülke's lab
• GP3308 (ΔyugI::tet), available in Jörg Stülke's lab
• GP3309 (ΔyugI::tet) (ΔyabR::aphA3), available in Jörg Stülke's lab
• MGNA-A620 (yugI::erm), available at the NBRP B. subtilis, Japan
• BKE31390 (yugI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATAACAACTCCTAAG, downstream forward: _UP4_TAAGCATGAAAAAAGCACCG
• BKK31390 (yugI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATAACAACTCCTAAG, downstream forward: _UP4_TAAGCATGAAAAAAGCACCG
• GP3322 (yugI-His spec), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP3700 (spc, based on pGP1331), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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