Difference between revisions of "PBQ200"

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'''Application:'''
 
'''Application:'''
  
The vector was constructed in the G. Rapoport lab and it is suitable for overexpression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pBQ200 is based on the vector pHT315.
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The vector was constructed in the George Rapoport lab and it is suitable for constitutive overexpression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. [[pBQ200]] is based on the vector pHT315. A Shine-Dalgarno sequence has to be fused to the open reading frame during PCR.
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Sequencing primers:
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*'''M13_puc_for:'''                      5‘-GTAAAACGACGGCCAGTG-3‘
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*'''M13_puc_rev:'''                      5‘-GGAAACAGCTATGACCATG-3‘
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*'''FX125 (rev; primes -85bp of MCS):''' 5‘-GGCTCGTATGTTGTGTGG-3‘
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*'''JN54 (fwd; primes +63bp of MCS):''' 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘
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'''Reference:'''
 
'''Reference:'''
 
<pubmed> 8057358 </pubmed>
 
<pubmed> 8057358 </pubmed>
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==  Return to [[Plasmids]] ==

Latest revision as of 08:12, 29 July 2014

pBQ200: click to enlarge

Application:

The vector was constructed in the George Rapoport lab and it is suitable for constitutive overexpression of proteins in B. subtilis. The plasmid confers resistance to ampicillin and erythromycin in E. coli and B. subtilis, respectively. pBQ200 is based on the vector pHT315. A Shine-Dalgarno sequence has to be fused to the open reading frame during PCR.

Sequencing primers:

  • M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
  • M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
  • FX125 (rev; primes -85bp of MCS): 5‘-GGCTCGTATGTTGTGTGG-3‘
  • JN54 (fwd; primes +63bp of MCS): 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘


Reference:

Return to Plasmids