mtnX

168

2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase

Locus: BSU_13600

Molecular weight: 26.85 kDa

Isoelectric point: 4.66

Protein length: 235 aa

Gene length: 708 bp

Function: methionine salvage

Product: 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase

Essential: no

Synonyms: mtnX, ykrX

Genomic Context

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List of homologs in different organisms, belongs to COG4359 (Galperin et al., 2021)

Gene

Coordinates: 1,428,275  1,428,982

The protein

Catalyzed reaction/ biological activity

• 2-hydroxy-5-methylsulfanyl-3-oxopent-1-enyl phosphate + H₂O --> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate (according to UniProt)

Protein family

• HAD superfamily (according to UniProt)

Structure

• 2FEA (PDB) PubMed
• O31667 (AlphaFold)

Expression and Regulation

Operons

• Genes: mtnW-mtnX-mtnB-mtnD

  Description: PubMed

  Regulation

  • induced by methionine starvation (S-box) PubMed

  Regulatory mechanism

  • S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A785 (ykrX::erm), available at the NBRP B. subtilis, Japan
• BKE13600 (mtnX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATAATAAAAGGTTTTCGAG,  downstream forward: _UP4_GAGAATGTAAAGGAGGTACA
• BKK13600 (mtnX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATAATAAAAGGTTTTCGAG,  downstream forward: _UP4_GAGAATGTAAAGGAGGTACA

References

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