sdhC

168

succinate dehydrogenase (cytochrome b558 subunit)

Locus: BSU_28450

Molecular weight: 22.79 kDa

Isoelectric point: 9.83

Protein length: 202 aa

Gene length: 609 bp

Function: TCA cycle

Product: succinate dehydrogenase (cytochrome b558 subunit)

Essential: no

Synonyms: sdhC

Genomic Context

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List of homologs in different organisms, belongs to COG2009 (Galperin et al., 2021)

Gene

Coordinates: 2,908,129  2,908,737

Phenotypes of a mutant

• defective in the the ability to support the growth of Synechococcus leopoliensis CCAP1405/1 on agar media PubMed
• defective in biofilm formation PubMed

The protein

Protein family

• cytochrome b558 family (single member, according to UniProt)

Cofactors

• 2 protoheme IX molecules (heme b_H and heme b₅₅₈) PubMed

Structure

• 1NEK (PDB) (E. coli) PubMed
• P08064 (AlphaFold)

Effectors of protein activity

• Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide PubMed
• Activated by Cytochrome b558 PubMed

Localization

• membrane protein PubMed

Expression and Regulation

Operons

• Genes: sdhC-sdhA-sdhB

  Description: PubMed

  Regulation

  • constitutive

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Other regulations

• FsrA: translation inhibition, PubMed

Additional information

• highly expressed at high iron concentrations PubMed

Biological materials

Mutant

• MGNA-B028 (sdhC::erm), available at the NBRP B. subtilis, Japan
• GP743 ∆(sdhC-sdhA)::cat, available in Jörg Stülke's lab
• GP792 ∆(sdhC-sdhA-sdhB)::phleo, available in Jörg Stülke's lab
• GP2342 ∆(sdhC-sdhA-sdhB)::kan, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
• GP2343 ∆(sdhC-sdhA-[[gene|sdhB]')::lox72, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
• BKE28450 (∆sdhC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACTTTACCCCCTGTTT,  downstream forward: _UP4_TAAGAGTACTAGATTACTAG
• BKK28450 (∆sdhC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACTTTACCCCCTGTTT,  downstream forward: _UP4_TAAGAGTACTAGATTACTAG

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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