ybdK

168

two-component sensor kinase

Locus: BSU_02010

Molecular weight: 37.12 kDa

Isoelectric point: 5.21

Protein length: 320 aa

Gene length: 963 bp

Function: unknown

Product: two-component sensor kinase

Essential: no

E.C.: null

Synonyms: ybdK

Genomic Context

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List of homologs in different organisms, belongs to COG0642 (Galperin et al., 2021)

Gene

Coordinates: 221,950 → 222,912

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of YbdJ
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

• two transmembrane segments
• HAMP domain (aa 67-120) (according to UniProt)
• Histidine kinase domain (aa 135-320) (according to UniProt)

Structure

• O31433 (AlphaFold)

Modification

• autophosphorylation on a His residue

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ybdK

  Description: DBTBS

  Regulatory mechanism

  • ComK: activation, in comK regulon

Biological materials

Mutant

• MGNA-B957 (ybdK::erm), available at the NBRP B. subtilis, Japan
• BKE02010 (ΔybdK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACTGATATTTTGTCTTGA,  downstream forward: _UP4_TGACCCCGCTGATGTTTTTC
• BKK02010 (ΔybdK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACTGATATTTTGTCTTGA,  downstream forward: _UP4_TGACCCCGCTGATGTTTTTC

References

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