pstBB

168

phosphate ABC transporter (ATP-binding protein)

Locus: BSU_24950

Molecular weight: 29.05 kDa

Isoelectric point: 7.77

Protein length: 260 aa

Gene length: 783 bp

Function: high-affinity phosphate uptake

Product: phosphate ABC transporter (ATP-binding protein)

Essential: no

E.C.: 3.6.3.27

Synonyms: pstBB, pstB, yqgK, yzmF

Genomic Context

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List of homologs in different organisms, belongs to COG1117 (Galperin et al., 2021)

Gene

Coordinates: 2,577,210  2,577,992

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + phosphate --> ADP + H⁺ + 2 phosphate (according to UniProt)

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 8-255) (according to UniProt)

Structure

• 4YMS (PDB) (from Caldanaerobacter subterraneus, 38% identity) PubMed
• P46342 (AlphaFold)

Paralogous protein(s)

• PstBA, GlnQ, ArtR, TcyN, TcyC, YxeO

Expression and Regulation

Operons

• Genes: pstS-pstC-pstA-pstBA-pstBB

  Description: PubMed

  Regulation

  • expressed under conditions of phosphate limitation (5000-fold induced) (PhoP) PubMed

  Regulatory mechanism

  • PhoP: activation, PhoP-P, PubMed, in phoP regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • mRNA processing between pstS and pstC to maintain higher concentrations of PstS relative to other components of the ABC transporter PubMed

Biological materials

Mutant

• MGNA-C450 (yqgK::erm), available at the NBRP B. subtilis, Japan
• BKE24950 (pstBB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGTAGCAATACTCATTGCCG,  downstream forward: _UP4_TGAAAAAAACAGGCTGAGTC
• BKK24950 (pstBB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGTAGCAATACTCATTGCCG,  downstream forward: _UP4_TGAAAAAAACAGGCTGAGTC

Labs working on this gene/protein

• Marion Hulett, University of Illinois at Chicago, USA Homepage

References

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