yodU

168

5' part of the split gene ypqP (together with ypqP), legionaminic acid synthesis, in B. subtilis 168 the gene is disrupted by the SP-beta prophage

Locus: BSU_19810

Molecular weight: 15.00 kDa

Isoelectric point: 9.819

Protein length: 139 aa

Gene length: 420 bp

Function: legionaminic acid synthesis

Product: UDP-NAcGlcA inverting 4,6-dehydratase (N-terminal part)

Essential: no

Synonyms: yodU, ypqP, spsM

Genomic Context

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Gene

Coordinates: 2,151,626 → 2,152,045

Phenotypes of a mutant

• the ypqP mutant of PY79 has a less hydrophobic spore surface PubMed

The protein

Catalyzed reaction/ biological activity

• SpsM catalyzes the C-4/C-6 dehydration of the UDP-GlcNAc to produce UDP-4-keto-6-deoxy-GlcNAc, this is the first reaction of the legionaminic acid biosynthesis pathway PubMed
• required for spore crust assembly, legionaminic acid synthesis PubMed

Domains

• SpsM: Polysacc_synt_2 domain (Pfam accession number, PF02719) in the 18–296-aa region PubMed

Structure

• 2GN4 (PDB) (from Helicobacter pylori, 46% identity) PubMed

Paralogous protein(s)

• EpsC, (for SpsM)

Localization

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yodU

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: activation, PubMed, in gerE regulon

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• MGNA-A060 (yodU::erm), available at the NBRP B. subtilis, Japan
• BKE19810 (ΔyodU::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCTTATCCTCCAACTC,  downstream forward: _UP4_GTATATTAAGATACTTACTA
• BKK19810 (ΔyodU::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCTTATCCTCCAACTC,  downstream forward: _UP4_GTATATTAAGATACTTACTA

References

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