yrdF

168

similar to ribonuclease inhibitor

Locus: BSU_26730

Molecular weight: 10.59 kDa

Isoelectric point: 3.94

Protein length: 91 aa

Gene length: 276 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yrdF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2732 (Galperin et al., 2021)

Gene

Coordinates: 2,730,560 → 2,730,835

The protein

Protein family

• barstar family (single member, according to UniProt)

Structure

• 1A19 (PDB) (from Bacillus amyloliquefaciens 49%identity)
• O07938 (AlphaFold)

Biological materials

Mutant

• BKE26730 (ΔyrdF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAGAAACTATGCCCTCC,  downstream forward: _UP4_TAGACTTTCTGTTTTGCGAA
• BKK26730 (ΔyrdF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAGAAACTATGCCCTCC,  downstream forward: _UP4_TAGACTTTCTGTTTTGCGAA

LacZ fusion

• pGP3807 (in pAC7), available in Jörg Stülke's lab PubMed

References

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