yoaI

168

similar to oxygenase component of 4-hydroxyphenylacetate 3-monooxygenase

Locus: BSU_18620

Molecular weight: 43.01 kDa

Isoelectric point: 5.13

Protein length: 483 aa

Gene length: 1452 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yoaI

Genomic Context

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List of homologs in different organisms, belongs to COG2368 (Galperin et al., 2021)

Gene

Coordinates: 2,031,439  2,032,890

The protein

Catalyzed reaction/ biological activity

• 4-hydroxyphenylacetate + FADH₂ + O₂ --> 3,4-dihydroxyphenylacetate + FAD + H⁺ + H₂O (according to UniProt)

Protein family

• FADH(2)-utilizing monooxygenase family (single member, according to UniProt)

Cofactors

• FAD (according to UniProt)

Structure

• 2YYG (PDB) (from Thermus thermophilus, 42% identity) PubMed
• C0SPC0 (AlphaFold)

Expression and Regulation

Operons

• Genes: yoaI

  Description: PubMed

Biological materials

Mutant

• MGNA-A838 (yoaI::erm), available at the NBRP B. subtilis, Japan
• BKE18620 (yoaI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTAAGTTTCGCTCCTTC,  downstream forward: _UP4_TGAAAAAACAGCGGGAAAAG
• BKK18620 (yoaI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTAAGTTTCGCTCCTTC,  downstream forward: _UP4_TGAAAAAACAGCGGGAAAAG

References

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