yoaE

168

formate dehydrogenase

Locus: BSU_18570

Molecular weight: 76.06 kDa

Isoelectric point: 7.33

Protein length: 680 aa

Gene length: 2043 bp

Function: unknown

Product: formate dehydrogenase

Essential: no

Synonyms: yoaE

Genomic Context

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List of homologs in different organisms, belongs to COG0243 (Galperin et al., 2021)

Gene

Coordinates: 2,025,400  2,027,442

The protein

Protein family

• prokaryotic molybdopterin-containing oxidoreductase family (according to UniProt)

Domains

• 4Fe-4S Mo/W bis-MGD-type domain (aa 9-66) (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 2VPW (PDB) (from Thermus thermophilus, 25% identity) PubMed
• C0SP82 (AlphaFold)

Paralogous protein(s)

• YyaE

Expression and Regulation

Operons

• Genes: yoaE-yoaF

  Description: PubMed

Biological materials

Mutant

• MGNA-A836 (yoaE::erm), available at the NBRP B. subtilis, Japan
• BKE18570 (yoaE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCCCAGTTCCTCTCTC,  downstream forward: _UP4_TGAAAAATAATGAAACCCGG
• BKK18570 (yoaE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCCCAGTTCCTCTCTC,  downstream forward: _UP4_TGAAAAATAATGAAACCCGG

References

Research papers

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