ywcB

168

unknown

Locus: BSU_38230

Molecular weight: 11.58 kDa

Isoelectric point: 9.94

Protein length: 102 aa

Gene length: 309 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: ywcB, ipa-32r

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3162 (Galperin et al., 2021)

Gene

Coordinates: 3,923,920  3,924,228

Phenotypes of a mutant

• ywcBA mutant has slightly reduced acetic acid transport PubMed
• ywcBA mutant shows earlier pellicle biofilm formation PubMed

The protein

Structure

• P39600 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ywcB-ywcA

  Regulation

  • expressed during sporulation (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• BKE38230 (ywcB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCTTTCCTCCTTTTAT,  downstream forward: _UP4_GAGGATCTGAAGGGTGAACA
• BKK38230 (ywcB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCTTTCCTCCTTTTAT,  downstream forward: _UP4_GAGGATCTGAAGGGTGAACA

References

Chen Y, Gozzi K, Yan F, Chai Y Acetic Acid Acts as a Volatile Signal To Stimulate Bacterial Biofilm Formation. mBio. 2015 Jun 09; 6(3):e00392. doi:10.1128/mBio.00392-15. pii:e00392-15. PMID:26060272
Steil L, Serrano M, Henriques AO, Völker U Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis. Microbiology (Reading, England). 2005 Feb; 151(Pt 2):399-420. . PMID:15699190