yisZ

168

adenylylsulfate kinase

Locus: BSU_10910

Molecular weight: 22.16 kDa

Isoelectric point: 7.21

Protein length: 199 aa

Gene length: 600 bp

Function: sulfate reduction and activation

Product: adenylylsulfate kinase

Essential: no

E.C.: 2.7.1.25

Synonyms: yisZ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0529 (Galperin et al., 2021)

Gene

Coordinates: 1,169,877 → 1,170,476

The protein

Catalyzed reaction/ biological activity

• adenosine 5'-phosphosulfate + ATP --> 3'-phosphoadenylyl sulfate + ADP + H⁺ (according to UniProt)

Protein family

• APS kinase family (with CysC, according to UniProt)

Structure

• 3CR8 (PDB) (from Escherichia coli, 38% identity, 55% similarity) PubMed
• O06735 (AlphaFold)

Expression and Regulation

Operons

• Genes: yitB-yitA-yisZ

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigK) PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• MGNA-A499 (yisZ::erm), available at the NBRP B. subtilis, Japan
• BKE10910 (ΔyisZ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCAAATGATGTTCGGATTGT,  downstream forward: _UP4_TAAAAAACCCGATCAATGTC
• BKK10910 (ΔyisZ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCAAATGATGTTCGGATTGT,  downstream forward: _UP4_TAAAAAACCCGATCAATGTC

References

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