secA

168

preprotein translocase subunit (ATPase), required for membrane targeting of DivIVA, motor protein that drives preprotein translocation through the SecY-SecE-SecG channel

Locus: BSU_35300

Molecular weight: 95.33 kDa

Isoelectric point: 5.34

Protein length: 841 aa

Gene length: 2526 bp

Function: protein secretion

Product: preprotein translocase subunit (ATPase)

Essential: yes

Synonyms: secA, div, div-341, ts-341

Genomic Context

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List of homologs in different organisms, belongs to COG0653 (Galperin et al., 2021)

Gene

Coordinates: 3,628,310  3,630,835

Phenotypes of a mutant

• essential PubMed
• terminal phenotype of CRISPRi knockdown mutant is moderate bending and a cessation of growth PubMed

The protein

Catalyzed reaction/ biological activity

• ATP  ADP + Pi + preprotein translocation
• required for membrane targeting of DivIVA PubMed

Protein family

• SecA family (single member, according to UniProt)

Domains

• nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain

Cofactors

• Mg

Structure

• 1TF5 (PDB) (open structure)
• 2IBM (PDB)
• 3DL8 (PDB) (structure of the (SecY-SecE-SecG)-SecA complex PubMed
• P28366 (AlphaFold)

Effectors of protein activity

• anionic phospholipids, preprotein, SecY, signal peptides (even when added in trans) PubMed

Localization

• mainly in the cytoplasm PubMed
• a small fraction is statically associated with the cell membrane PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: secA-prfB

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BEC35300, a CRISPRi knockdown mutant, is available from the Bacillus Genetic Stock Center

References

Reviews

Tsirigotaki A, De Geyter J, Šoštaric N, Economou A, Karamanou S Protein export through the bacterial Sec pathway. Nature reviews. Microbiology. 2017 Jan; 15(1):21-36. doi:10.1038/nrmicro.2016.161. PMID:27890920
Collinson I SecA-a New Twist in the Tale. Journal of bacteriology. 2017 Jan 15; 199(2). pii:e00736-16. doi:10.1128/JB.00736-16. PMID:27799326
Kang Z, Yang S, Du G, Chen J Molecular engineering of secretory machinery components for high-level secretion of proteins in Bacillus species. Journal of industrial microbiology & biotechnology. 2014 Nov; 41(11):1599-607. doi:10.1007/s10295-014-1506-4. PMID:25212246

Original Publications

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