menE

168

o-succinylbenzoate-CoA ligase

Locus: BSU_30790

Molecular weight: 54.02 kDa

Isoelectric point: 5.47

Protein length: 486 aa

Gene length: 1461 bp

Function: biosynthesis of menaquinone

Product: o-succinylbenzoate-CoA ligase

Essential: yes

E.C.: 6.2.1.26

Synonyms: menE

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Metabolism, Additional metabolic pathways, Biosynthesis of cofactors, Biosynthesis of menaquinone

Groups of genes, Essential genes

List of homologs in different organisms, belongs to COG0318 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, SigB regulon

Gene

Coordinates: 3,147,350  3,148,810

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

ATP + 2-succinylbenzoate + CoA --> AMP + diphosphate + 2-succinylbenzoyl-CoA PubMed

Protein family

ATP-dependent AMP-binding enzyme family (according to UniProt)

Structure

5BUQ (PDB) PubMed
5X8F (PDB) (complex with AMP and product analog) PubMed
5X8G (PDB) (complex with product analog) PubMed
P23971 (AlphaFold)

Modification

MenE is acetylated on Lys-471 by AcuA, acetylation results in inactivation of MenE PubMed
deacetylation is catalyzed by SrtN PubMed

Expression and Regulation

Operons

Genes: menE-menC

Description: PubMed

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon
SigB: sigma factor, PubMed, in sigB regulon

Additional information

A ncRNA is predicted between 'mntA' and 'menC' PubMed

Genes: menB-menE-menC

Description: PubMed

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Additional information

A ncRNA is predicted between 'mntA' and 'menC' PubMed

Genes: menF-menD-ytxM-menB-menE-menC

Description: PubMed

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Additional information

A ncRNA is predicted between 'mntA' and 'menC' PubMed

References

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