comGD

168

DNA transport machinery, minor pseudopilin

Locus: BSU_24700

Molecular weight: 15.87 kDa

Isoelectric point: 9.88

Protein length: 143 aa

Gene length: 432 bp

Function: genetic competence, DNA uptake

Product: minor pseudopilin

Essential: no

Synonyms: comGD

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,557,252 → 2,557,683

The protein

Structure

• P25956 (AlphaFold)

Localization

• associated to the membrane via ComGG PubMed
• cell surface PubMed

Expression and Regulation

Operons

• Genes: comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL

  Description: PubMed

  Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigF: sigma factor, PubMed, in sigF regulon

  Additional information

  • the ''comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL'' operon requires NusA for expression Reference

Biological materials

Mutant

• BKE24700 (ΔcomGD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTTAATGTTCAACCTTAAC,  downstream forward: _UP4_GTCAATGTGGAGAGAAAATA
• BKK24700 (ΔcomGD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTTAATGTTCAACCTTAAC,  downstream forward: _UP4_GTCAATGTGGAGAGAAAATA
• BP914 (Δ(comGA-comGB-comGC-comGD-comGE-comGF-comGG)::phleo), available in Fabian Commichau's lab

References

Reviews

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Original Publications

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