yhdW

168

similar to glycerophosphodiester phosphodiesterase

Locus: BSU_09620

Molecular weight: 27.33 kDa

Isoelectric point: 9.54

Protein length: 243 aa

Gene length: 732 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yhdW

Genomic Context

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List of homologs in different organisms, belongs to COG0584 (Galperin et al., 2021)

Gene

Coordinates: 1,037,688  1,038,419

The protein

Catalyzed reaction/ biological activity

• sn-glycero-3-phosphoester + H₂O --> alcohol + H⁺ + sn-glycerol 3-phosphate (according to UniProt)

Protein family

• glycerophosphoryl diester phosphodiesterase family (with GlpQ, according to UniProt)

Domains

• GP-PDE domain (aa 1-238) (according to UniProt)

Structure

• 5T9B (PDB) (GlpQ, 38% identity) PubMed
• O07592 (AlphaFold)

Paralogous protein(s)

• YqiK, GlpQ

Expression and Regulation

Operons

• Genes: yhdW-yhdV-fluC

  Description: PubMed

Biological materials

Mutant

• MGNA-B487 (yhdW::erm), available at the NBRP B. subtilis, Japan
• BKE09620 (yhdW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCTAAGTCAGCTCCTCG,  downstream forward: _UP4_GATTTCATCATAAAGGATGG
• BKK09620 (yhdW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCTAAGTCAGCTCCTCG,  downstream forward: _UP4_GATTTCATCATAAAGGATGG

References

Research papers

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