ywfO

168

putative metal-dependent phosphohydrolase

locus: BSU_37600

Molecular weight: 50.81 kDa

pI: 5.84

Protein length: 433 aa

Gene length: 1302 bp

function: unknown

product: unknown

essential: no

synonyms: ywfO, ipa-93d

List of homologs in different organisms, belongs to COG1078 (Galperin et al., 2021)

Gene

Coordinates: 3,859,535  3,860,836

The protein

[wiki|Domains]

• [wiki|HD domain] (aa 61-178) (according to UniProt)

Structure

• [PDB|2O6I] (from Enterococcus faecalis, 50% identity) [pubmed|21757692]
• [AF|P39651]

Expression and Regulation

Operons

• genes: [gene|1EC8C3FC0B3BAC12CB7C5C0E1F4886E198AA1D53|ywzC]-[gene|268A50A41FB6CAB0D768201996C5DF5DADCCA0B7|ywfO]-[gene|BD23EC2EAC16D3A4BCAE19222E29C2F8AEB30AA6|ywgA]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A585 (ywfO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/585 NBRP B. subtilis, Japan]
• BKE37600 ([gene|268A50A41FB6CAB0D768201996C5DF5DADCCA0B7|ywfO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGTCATTCCCTCTTTTT,  downstream forward: _UP4_TAGAAATTGGAAAGAAGGAA
• BKK37600 ([gene|268A50A41FB6CAB0D768201996C5DF5DADCCA0B7|ywfO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGTCATTCCCTCTTTTT,  downstream forward: _UP4_TAGAAATTGGAAAGAAGGAA

References

Research papers

• 21757692