ywbG

168

holin-like auxilary protein

Locus: BSU_38330

Molecular weight: 25.67 kDa

Isoelectric point: 9.56

Protein length: 225 aa

Gene length: 678 bp

Function: unknown

Product: holin-like auxilary protein

Essential: no

Synonyms: ywbG, ipa-22r

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1346 (Galperin et al., 2021)

Gene

Coordinates: 3,933,577  3,934,254

Phenotypes of a mutant

• a ''ywbH-ywbG'' mutant is delayed in pellicle formation PubMed
• a ''ywbH-ywbG yxaK-yxaC pftA-pftB'' triple mutant is severely delayed in pellicle formation PubMed
• a ''ywbH-ywbG'' overexpression strain has more robust pellicle formation PubMed

The protein

Protein family

• YohK (E.coli)/YwbG (IPA-22R) (B.subtilis) family (with YxaC, according to UniProt)

Structure

• P39590 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ywbH-ywbG

  Description: PubMed

  Regulation

  • induced by acetate (YwbI) PubMed

  Regulatory mechanism

  • YwbI: activation, PubMed, in ywbI regulon

Biological materials

Mutant

• MGNA-B224 (ywbG::erm), available at the NBRP B. subtilis, Japan
• BKE38330 (ywbG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACTTACAATTCCGATGAACA,  downstream forward: _UP4_TAAAAAACTGTCCTCTCGAG
• BKK38330 (ywbG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACTTACAATTCCGATGAACA,  downstream forward: _UP4_TAAAAAACTGTCCTCTCGAG

References

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