ganS

168

galactotriose ABC transporter, binding protein

Locus: BSU_34160

Molecular weight: 45.38 kDa

Isoelectric point: 5.44

Protein length: 421 aa

Gene length: 1266 bp

Function: uptake of galactotriose

Product: galactotriose ABC transporter, binding protein

Essential: no

Synonyms: cycB, yvfK

Genomic Context

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List of homologs in different organisms, belongs to COG2182 (Galperin et al., 2021)

Gene

Coordinates: 3,507,253 → 3,508,518

Phenotypes of a mutant

• a ''ganS-ganP-ganQ-ganA-ganB'' mutant is impaired in the utilization of galactan PubMed

The protein

Protein family

• Bacterial solute-binding protein 1 family (according to UniProt)

Structure

• 2ZYK (PDB) (from Thermoactinomyces vulgaris, 38% identity) PubMed
• O07009 (AlphaFold)

Paralogous protein(s)

• MdxE

Localization

• cell membrane (via GanP-GanQ) PubMed

Expression and Regulation

Operons

• Genes: ganS-ganP-ganQ-ganA-ganB

  Description: PubMed

  Regulation

  • induced by galactan (binding of galactobiose to GanR) PubMed
  • repressed by glucose (CcpA) PubMed

  Regulatory mechanism

  • GanR: repression, PubMed, in ganR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A484 (yvfK::erm), available at the NBRP B. subtilis, Japan
• BKE34160 (ΔganS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATTGTCACCCTTTCG,  downstream forward: _UP4_TAAAGAGAAAGCTAGATTTG
• BKK34160 (ΔganS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATTGTCACCCTTTCG,  downstream forward: _UP4_TAAAGAGAAAGCTAGATTTG

References

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