yuiF

168

membrane protein, similar to histidine transporter

Locus: BSU_32040

Molecular weight: 45.77 kDa

Isoelectric point: 5.93

Protein length: 442 aa

Gene length: 1329 bp

Function: uptake of histidine

Product: potential histidine transporter

Essential: no

E.C.: null

Synonyms: yuiF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2056 (Galperin et al., 2021)

Gene

Coordinates: 3,294,942  3,296,270

The protein

Catalyzed reaction/ biological activity

• uptake of histidine (based on similarity to the orthologue in Staphylococcus aureus bioRxiv)

Structure

• O32105 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yuiF

  Description: PubMed

Biological materials

Mutant

• MGNA-B568 (yuiF::erm), available at the NBRP B. subtilis, Japan
• BKE32040 (yuiF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGATACCCCTCCAAA,  downstream forward: _UP4_TAGGAAAAAGGCTCCCCTTT
• BKK32040 (yuiF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGATACCCCTCCAAA,  downstream forward: _UP4_TAGGAAAAAGGCTCCCCTTT

References

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