nadR

168

transcriptional repressor of genes involved in NAD biosynthesis

Locus: BSU_27890

Molecular weight: 19.58 kDa

Isoelectric point: 5.76

Protein length: 180 aa

Gene length: 543 bp

Function: regulation of NAD biosynthesis

Product: transcriptional repressor

Essential: no

Synonyms: nadR, yrxA

Genomic Context

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List of homologs in different organisms, belongs to COG1827 (Galperin et al., 2021)

Gene

Coordinates: 2,850,716  2,851,258

The protein

Structure

• 1J5Y (PDB) (from Thermotoga maritima, 38% identity) PubMed
• P39667 (AlphaFold)

Expression and Regulation

Operons

• Genes: nifS-nadR

  Description: PubMed

  Regulation

  • repressed in the presence of nicotinic acid (NadR) PubMed

  Regulatory mechanism

  • NadR: repression, PubMed, in nadR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A824 (yrxA::erm), available at the NBRP B. subtilis, Japan
• BKE27890 (nadR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCCTTCAGCCACAGAAGAA,  downstream forward: _UP4_TAAAAAAAGCCCTCTAGTGG
• BKK27890 (nadR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCCTTCAGCCACAGAAGAA,  downstream forward: _UP4_TAAAAAAAGCCCTCTAGTGG

Labs working on this gene/protein

• Alessandra Albertini, University of Pavia, Italy homepage

References

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