yqkF

168

NADPH-dependent 4-Hydroxy-2,3-trans-nonenal reductase

Locus: BSU_23620

Molecular weight: 34.56 kDa

Isoelectric point: 5.13

Protein length: 306 aa

Gene length: 921 bp

Function: unknown

Product: NADPH-dependent 4-Hydroxy-2,3-trans-nonenal reductase

Essential: no

Synonyms: yqkF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0667 (Galperin et al., 2021)

Gene

Coordinates: 2,459,326 → 2,460,246

The protein

Protein family

• Aldo/keto reductase family (according to UniProt)
• Aldo/keto reductase 2 subfamily (according to UniProt)

Cofactors

• NADPH PubMed

Structure

• 1YNP (PDB) (from B. halodurans, 64% identity) PubMed
• P54569 (AlphaFold)

Paralogous protein(s)

• IolS, (31,4%)

Expression and Regulation

Operons

• Genes: yqkF

  Description: (according to DBTBS) null

• Genes: mciZ-yqkF

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• BKE23620 (ΔyqkF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGCACACTCCTTTTTT,  downstream forward: _UP4_TAGCAGAAAAACCAGCACCT
• BKK23620 (ΔyqkF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGCACACTCCTTTTTT,  downstream forward: _UP4_TAGCAGAAAAACCAGCACCT