aimR

168

transcriptional antiterminator, allows expression of aimX, part of the arbitrium peptide-based communication system, phage SP beta lytic/lysogenic regulator

Locus: BSU_20860

Molecular weight: 45.16 kDa

Isoelectric point: 5.86

Protein length: 386 aa

Gene length: 1161 bp

Function: control of phage SP beta lysogeny

Product: SP beta arbitrium transcription factor

Essential: no

Synonyms: aimR, yopK

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,208,994 → 2,210,154

Phenotypes of a mutant

• reduced number of phage particles produced after prophage induction, this can be suppressed by inactivation of yopN PubMed

The protein

Catalyzed reaction/ biological activity

• activates aimX expression in the apo form (in the absence of AimP) PubMed
• binds to the aimX mRNA upstream region to act as antiterminator PubMed

Structure

• Apo-form: 5XYB (PDB) (PubMed), 5ZW5 (PDB) (PubMed), 6IPX (PDB) (PubMed), 6HP3 (PDB)(PubMed), 6JG5 (PDB) (PubMed)
• Complexed with AimP hexapeptide (AimP): 5Y24 (PDB) (PubMed), 5ZW6 (PDB) (PubMed), 6IM4 (PDB) (PubMed), 6HP5 (PDB) (PubMed), 6JG9 (PDB) (PubMed)
• Complexed with DNA: 6HP7 (PDB) (PubMed), 6JG8 (PDB) (PubMed)
• O31927 (AlphaFold)

Expression and Regulation

Operons

• Genes: aimR-aimP

  Description

  • PubMed

  Expression

  • repressed by MrpR

  Regulatory mechanism

  • MrpR: repression, in mrpR regulon

Biological materials

Mutant

• BKE20860 (ΔaimR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTTCCCCTCATTTCT,  downstream forward: _UP4_TAAAGGAGGTGAGACAATGA
• BKK20860 (ΔaimR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTTCCCCTCATTTCT,  downstream forward: _UP4_TAAAGGAGGTGAGACAATGA

References

Research papers

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