purB

purB
168

adenylsuccinate lyase

Locus
BSU_06440
Molecular weight
49.32 kDa
Isoelectric point
5.82
Protein length
Gene length
Function
purine biosynthesis
Product
adenylsuccinate lyase
Essential
no
E.C.
4.3.2.2
Synonyms
purB

Genomic Context

List of homologs in different organisms, belongs to COG0015 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
700,232 701,527
Phenotypes of a mutant
poor growth PubMed
non-transformable PubMed
The protein
Catalyzed reaction/ biological activity
N6-(1,2-dicarboxyethyl)-AMP --> AMP + fumarate (according to UniProt)
(2S)-2-[5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamido]succinate --> 5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamide + fumarate (according to UniProt)
Protein family
lyase 1 family (according to UniProt)
Structure
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Description
Regulation
expression activated by glucose (4.4 fold) PubMed
Regulatory mechanism
G-box: RNA switch, (riboswitch) PubMed, in G-box
PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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purEpurD

2025-07-16 17:01:58

Jstuelk

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ace69dc80f4f087486c218727fa370a7a9630d21

EDD8EC37B0E5B38941D5827C265AB2F23CF23EA7

Biological materials
Mutant
BKE06440 (purB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC
BKK06440 (purB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC
References
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Page visits: 6236

Time of last update: 2025-07-19 03:15:31

Author of last update: Melvin.boenninger