dinG

168

ATP-dependent DNA helicase

Locus: BSU_22400

Molecular weight: 105.83 kDa

Isoelectric point: 5.82

Protein length: 931 aa

Gene length: 2796 bp

Function: response to DNA damage

Product: ATP-dependent DNA helicase

Essential: no

Synonyms: dinG

Genomic Context

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List of homologs in different organisms, belongs to COG2176 (Galperin et al., 2021)

Gene

Coordinates: 2,349,671  2,352,466

The protein

Catalyzed reaction/ biological activity

• binds to abasic and RNA base DNA lesions PubMed

Protein family

• helicase family (according to UniProt)

Domains

• Exonuclease domain (aa 7-162) (according to UniProt)
• Helicase ATP-binding domain (aa 250-510) (according to UniProt)
• Helicase C-terminal domain (aa 741-897) (according to UniProt)

Structure

• 4A15 (PDB) (from Thermoplasma acidophilum, corresponds to aa 257 ... 913 of DinG, 22% identity) PubMed
• P54394 (AlphaFold)

Paralogous protein(s)

• YpvA

Localization

• Cytoplasm (Homogeneous) PubMed

Expression and Regulation

Operons

• Genes: panB-panC-panD-dinG-ypmA-tseB-aspB

  Description: PubMed

Biological materials

Mutant

• MGNA-A423 (dinG::erm), available at the NBRP B. subtilis, Japan
• BKE22400 (dinG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAAATGGACACCTCAA,  downstream forward: _UP4_TAAAAGCCGCATAAAGCGGC
• BKK22400 (dinG::kan  trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_CATTAAAATGGACACCTCAA,  downstream forward: _UP4_TAAAAGCCGCATAAAGCGGC

References

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