cwlC

cwlC
168

N-acetylmuramoyl-L-alanine amidase

Locus
BSU_17410
Molecular weight
27.01 kDa
Isoelectric point
5.94
Protein length
Gene length
Function
mother cell lysis
Product
N-acetylmuramoyl-L-alanine amidase
Essential
no
E.C.
3.5.1.28
Synonyms
cwlC

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3087 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,872,812  1,873,579
The protein
Catalyzed reaction/ biological activity
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)
Protein family
contains an amidase_3 domain (like CwlD, LytC, YqiI, YrvJ)
MurNAc-LAA domain (aa 4-172) (according to UniProt)
SPOR domain (aa 180-254) (according to UniProt)
Structure
1X60 (PDB) (peptidoglycan binding domain) PubMed
secreted (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
Description
Regulation
expressed late during sporulation in the mother cell (SigK) PubMed
Sigma factors
SigK: sigma factor, PubMed, in sigK regulon
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cwlC

2025-01-17 22:57:29

ghost

66

0e02ed9e7225ca6d75c53bf0aa6c9f3afe8ef0b7

EE6F9C98C5E7AF7F0B89AB63FE9FB80F27DFC27B

Biological materials
Mutant
BKE17410 (cwlC::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTTTTCATCACCTCATTA,  downstream forward: _UP4_TAGCCGAGACGGGGACGAGC
BKK17410 (cwlC::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTTTTCATCACCTCATTA,  downstream forward: _UP4_TAGCCGAGACGGGGACGAGC
References
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Time of last update: 2025-02-17 15:36:34

Author of last update: Jstuelk