PbpF

• Description: penicillin-binding protein 2C
  
  

Gene name	pbpF
Synonyms	ponA
Essential	no
Product	penicillin-binding protein 2C
Function	bifunctional glucosyltransferase/ transpeptidase
MW, pI	79 kDa, 7.078
Gene length, protein length	2142 bp, 714 aa
Immediate neighbours	yhgC, hemE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU10110

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), prespore Link to a summary

Database entries

• Structure:

• Swiss prot entry: P38050

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor: SigA, SigG Link to a summary

• Regulation: expressed in mother cell and prespore Link to a summary

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

• Antibody:

References

D L Popham, P Setlow
Phenotypes of Bacillus subtilis mutants lacking multiple class A high-molecular-weight penicillin-binding proteins.
J Bacteriol: 1996, 178(7);2079-85
[PubMed:8606187] [WorldCat.org] [DOI] (P p)

D L Popham, P Setlow
Cloning, nucleotide sequence, and regulation of the Bacillus subtilis pbpF gene, which codes for a putative class A high-molecular-weight penicillin-binding protein.
J Bacteriol: 1993, 175(15);4870-6
[PubMed:8335642] [WorldCat.org] [DOI] (P p)