Difference between revisions of "NrgA"
| Line 93: | Line 93: | ||
* '''Regulation:''' induced by ammonium limitation | * '''Regulation:''' induced by ammonium limitation | ||
| + | ** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed] | ||
* '''Regulatory mechanism:''' transcription activation by [[TnrA]] | * '''Regulatory mechanism:''' transcription activation by [[TnrA]] | ||
| + | ** [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 17:40, 11 June 2009
- Description: ammonium transporter, required at low ammonium concentration
| Gene name | nrgA |
| Synonyms | amtB |
| Essential | no |
| Product | ammonium transporter |
| Function | ammonium uptake |
| MW, pI | 42 kDa, 5.727 |
| Gene length, protein length | 1212 bp, 404 aa |
| Immediate neighbours | ywoB, nrgB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU36510
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot), membrane
Database entries
- Structure:
- Swiss prot entry: Q07429
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: induced by ammonium limitation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Susan Fisher, Boston, USA homepage
Your additional remarks
References
Christian Detsch, Jörg Stülke
Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB.
Microbiology (Reading): 2003, 149(Pt 11);3289-3297
[PubMed:14600241]
[WorldCat.org]
[DOI]
(P p)
L V Wray, M R Atkinson, S H Fisher
The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein.
J Bacteriol: 1994, 176(1);108-14
[PubMed:8282685]
[WorldCat.org]
[DOI]
(P p)
M R Atkinson, S H Fisher
Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.
J Bacteriol: 1991, 173(1);23-7
[PubMed:1670935]
[WorldCat.org]
[DOI]
(P p)
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
