SigF

• Description: RNA polymerase forespore-specific (early) sigma factor SigF
  
  

Gene name	sigF
Synonyms	spoIIAC
Essential	no
Product	RNA polymerase forespore-specific (early) sigma factor SigF
Function	transcription of sporulation genes (late mother cell)
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	29 kDa, 5.078
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	spoVAA, spoIIAB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU23450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Genes controlled by SigF

dacF-spoIIAA-spoIIAB-sigF, sigG, pbpF, yphA-seaA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SigF-SpoIIAB

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1L0O (complex with SpoIIAB, Geobacillus stearothermophilus)

• Swiss prot entry: P07860

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dacF-spoIIAA-spoIIAB-sigF

spoIIAA-spoIIAB-sigF

• Sigma factor: dacF: SigF, SigG

spoIIAA: SigH

• Regulation: repressed by casamino acids PubMed , dacF: expressed during sporulation, spoIIAA: expressed early during sporulation
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism: spoIIAA: activated by Spo0A and repressed by SinR
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed