BglS

• Description: endo-beta-1,3-1,4 glucanase
  
  

Gene name	bglS
Synonyms	bgl, licS
Essential	no
Product	endo-beta-1,3-1,4 glucanase
Function	lichenan degradation
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	27 kDa, 6.482
Gene length, protein length	726 bp, 242 aa
Immediate neighbours	citH, licT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39070

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 16 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure:

• Swiss prot entry: P04957

• KEGG entry: [3]

• E.C. number: 3.2.1.73 3

Expression and regulation

• Operon:
  • licT-bglS PubMed
  • bglS PubMed

• Sigma factor: SigA

• Regulation: repressed by glucose (3.1-fold) (CcpA) PubMed1 PubMed2, expressed in the stationary phase (temporal activation), weak induction by ß-glucosides (LicT) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • LicT: binding to an RNA switch results in transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

K Schnetz, J Stülke, S Gertz, S Krüger, M Krieg, M Hecker, B Rak
LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family.
J Bacteriol: 1996, 178(7);1971-9
[PubMed:8606172] [WorldCat.org] [DOI] (P p)

S Krüger, J Stülke, M Hecker
Catabolite repression of beta-glucanase synthesis in Bacillus subtilis.
J Gen Microbiol: 1993, 139(9);2047-54
[PubMed:8245831] [WorldCat.org] [DOI] (P p)

J Stülke, R Hanschke, M Hecker
Temporal activation of beta-glucanase synthesis in Bacillus subtilis is mediated by the GTP pool.
J Gen Microbiol: 1993, 139(9);2041-5
[PubMed:8245830] [WorldCat.org] [DOI] (P p)

N Murphy, D J McConnell, B A Cantwell
The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase.
Nucleic Acids Res: 1984, 12(13);5355-67
[PubMed:6087283] [WorldCat.org] [DOI] (P p)

1. Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
2. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed

1. Murphy ND, McConnell DJ, Cantwell BA. 1984. The DNA sequence of the gene and genetic control sites for the excreted Bacillus subtilis enzyme β-glucanase. Nucl. Acids Res. 12:5355-5367. PubMed
2. Stülke, J., Hanschke, R. & Hecker, M. (1993) Temporal activation of ß-glucanase synthesis in Bacillus subtilis is mediated by the GTP pool. J. Gen. Microbiol. 139: 2041-2045. PubMed
3. Krüger, S., Stülke, J. & Hecker, M. (1993) Carbon catabolite repression of ß-glucanase synthesis in Bacillus subtilis. J. Gen. Microbiol. 139: 2047-2054. PubMed
4. Schnetz, K., Stülke, J., Gertz, S., Krüger, S., Krieg, M., Hecker, M. & Rak, B. (1996) LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family. J. Bacteriol. 178: 1971-1979. PubMed
5. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed