FbaA

• Description: fructose 1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme
  
  

Gene name	fbaA
Synonyms	fba, fba1, tsr
Essential	yes
Product	fructose-1,6-bisphosphate aldolase
Function	enzyme in glycolysis/ gluconeogenesis
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	30,2 kDa, 5.03
Gene length, protein length	855 bp, 285 amino acids
Immediate neighbours	spo0F, ywjH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37120

Phenotypes of a mutant

• Essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (according to Swiss-Prot)

• Protein family: class II fructose-bisphosphate aldolase family (according to Swiss-Prot)
• Paralogous protein(s): FbaB

Extended information on the protein

• Kinetic information: Reversible Michaelis-Menten PubMed

• Domains:
  • 2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)

• Modification: phosphorylation on Thr-212 and Thr-234 PubMed

• Cofactor(s): Zn2+ (Metalloenzyme)

• Effectors of protein activity:
  • Inhibited by alpha-ketoglutarate, oxaloacetate and pyruvate PubMed PubMed
  • Activated by NH4+ PubMed

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P13243

• KEGG entry: [3]

• E.C. number: 4.1.2.13

Additional information

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution

Expression and regulation

• Operon:
  • fbaA PubMed
  • fbaA-ywjH PubMed

• Sigma factor:

• Regulation: constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector: pGP395 (N-terminal His-tag, in pWH844), pGP88 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380)

• lacZ fusion: pGP601 (in pAC6)

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

1. Trach K, Chapman JW & Piggot P (1988) Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome J Bacteriol. 170: 4194-4208. PubMed
2. Ludwig H, Homuth G & Schmalisch M (2001) Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon Mol Microbiol. 41: 409-422. PubMed
3. Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed