Difference between revisions of "Mdh"
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29120] | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29120] | ||
− | * '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.37 1.1.1.37] | + | * '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.37 1.1.1.37] |
=== Additional information=== | === Additional information=== |
Revision as of 16:34, 10 June 2009
- Description: malate dehydrogenase
Gene name | mdh |
Synonyms | citH |
Essential | no |
Product | malate dehydrogenase |
Function | TCA cycle |
MW, pI | 33 kDa, 4.727 |
Gene length, protein length | 936 bp, 312 aa |
Immediate neighbours | icd, phoP |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU29120
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate + NAD+ = oxaloacetate + NADH (according to Swiss-Prot)
- Protein family: MDH type 3 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot), membrane associated PubMed
Database entries
- Structure: 1EMD (E.coli)
- Swiss prot entry: P49814
- KEGG entry: [3]
- E.C. number: 1.1.1.37
Additional information
- Sigma factor: SigA
mdh: constitutive
- Additional information:
Biological materials
- Mutant: GP719 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711]
[WorldCat.org]
[DOI]
(I p)