Difference between revisions of "CapB"

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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:'''
+
* '''Locus tag:''' BSU35900
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P96736 P96736]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P96736 P96736]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU35900 BSU35900]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU35900]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 13:37, 3 June 2009

  • Description: capsular polyglutamate biosynthesis

Gene name capB
Synonyms ywsC, pgsB
Essential no
Product poly-gamma-glutamate synthetase
Function capsule synthesis
MW, pI 43 kDa, 5.073
Gene length, protein length 1179 bp, 393 aa
Immediate neighbours capC, rbsR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwsC context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Locus tag: BSU35900

Phenotypes of a mutant

no synthesis of the poly-gamma-glutamate capsule PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: synthesis of poly-gamma-glutamate from L-Glu PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information: The operon is not expressed in the lab strain 168 due to lack of expression of DegQ resulting in inactivity of DegS and DegU

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Keitarou Kimura, Lam-Son Phan Tran, Thi-Huyen Do, Yoshifumi Itoh
Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto).
Biosci Biotechnol Biochem: 2009, 73(5);1149-55
[PubMed:19420703] [WorldCat.org] [DOI] (I p)

Cecilia Osera, Giuseppe Amati, Cinzia Calvio, Alessandro Galizzi
SwrAA activates poly-gamma-glutamate synthesis in addition to swarming in Bacillus subtilis.
Microbiology (Reading): 2009, 155(Pt 7);2282-2287
[PubMed:19389763] [WorldCat.org] [DOI] (P p)

Yuji Urushibata, Shinji Tokuyama, Yasutaka Tahara
Characterization of the Bacillus subtilis ywsC gene, involved in gamma-polyglutamic acid production.
J Bacteriol: 2002, 184(2);337-43
[PubMed:11751809] [WorldCat.org] [DOI] (P p)