Difference between revisions of "ArgF"

From SubtiWiki
Jump to: navigation, search
Line 35: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:'''
+
* '''Locus tag:''' BSU11250
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 80: Line 80:
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P18186 P18186]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P18186 P18186]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU11250 BSU11250]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU11250]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.1.3.3 2.1.3.3]
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.1.3.3 2.1.3.3]

Revision as of 13:34, 3 June 2009

  • Description: ornithine carbamoyltransferase

Gene name argF
Synonyms
Essential no
Product ornithine carbamoyltransferase
Function biosynthesis of arginine
MW, pI 34 kDa, 4.965
Gene length, protein length 957 bp, 319 aa
Immediate neighbours carB, yjzC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ArgF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU11250

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline (according to Swiss-Prot)
  • Protein family: ATCase/OTCase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: repressed by casamino acids PubMed , repressed by arginine (AhrC)
  • Regulatory mechanism: AhrC: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed