Difference between revisions of "DeaD"
(→Expression and regulation) |
|||
| Line 94: | Line 94: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[yxiM]]-[[deaD]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/10746760 PubMed] |
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 102: | Line 102: | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 15:31, 30 May 2009
- Description: DEAD-box RNA helicase
| Gene name | deaD |
| Synonyms | yxiN |
| Essential | no |
| Product | ATP-dependent RNA helicase |
| Function | RNA helicase |
| MW, pI | 53 kDa, 7.706 |
| Gene length, protein length | 1437 bp, 479 aa |
| Immediate neighbours | yxiO, yxiM |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: RNA helicase
- Protein family: DEAD-box RNA helicase
Extended information on the protein
- Kinetic information:
- Domains:
- Helicase domain 1: AS 1-203
- Linker region: AS 204-212
- Helicase domain 2: AS 207-368
- RNA-binding domain: AS 404-479
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Swiss prot entry:
- KEGG entry: BSU39110
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1121 (tet), available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage
Your additional remarks
References
- Caruthers, J. M., Hu, Y. & McKay, D. B. (2006). Structure of the second domain of the Bacillus subtilis DEAD-box RNA helicase YxiN. Acta Crystallogr Sect F Struct Biol Cryst Commun 62, 1191-1195.PubMed
- Karginov, F. V., Caruthers, J. M., Hu, Y., McKay, D. B. & Uhlenbeck, O. C. (2005). YxiN is a modular protein combining a DEx(D/H) core and a specific RNA-binding domain. J Biol Chem 280, 35499-35505.PubMed
- Karow, A. R., Theissen, B. & Klostermeier, D. (2007). Authentic interdomain communication in an RNA helicase reconstituted by expressed protein ligation of two helicase domains. FEBS J 274, 463-473.PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
