Difference between revisions of "CapB"
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| − | * '''Description:''' | + | * '''Description:''' capsular polyglutamate biosynthesis <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|''capB'' | |''capB'' | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywsC'' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywsC, pgsB'' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || poly-gamma-glutamate synthetase |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || capsule synthesis |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 5.073 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 5.073 | ||
| Line 40: | Line 40: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | |||
| + | no synthesis of the poly-gamma-glutamate capsule [http://www.ncbi.nlm.nih.gov/sites/entrez/11751809 PubMed] | ||
=== Database entries === | === Database entries === | ||
| Line 54: | Line 56: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' synthesis of poly-gamma-glutamate from L-Glu [http://www.ncbi.nlm.nih.gov/sites/entrez/11751809 PubMed] |
* '''Protein family:''' | * '''Protein family:''' | ||
| Line 90: | Line 92: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[capB]]-[[capC]]-[[capA]]'' |
* '''Sigma factor:''' | * '''Sigma factor:''' | ||
| Line 96: | Line 98: | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' activated by [[DegU]]-P |
| − | * '''Additional information:''' | + | * '''Additional information:''' The operon is not expressed in the lab strain 168 due to lack of expression of [[DegQ]] resulting in inactivity of [[DegS]] and [[DegU]] |
=Biological materials = | =Biological materials = | ||
| Line 112: | Line 114: | ||
* '''two-hybrid system:''' | * '''two-hybrid system:''' | ||
| − | * '''Antibody:''' | + | * '''Antibody:''' |
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
Revision as of 09:27, 21 May 2009
- Description: capsular polyglutamate biosynthesis
| Gene name | capB |
| Synonyms | ywsC, pgsB |
| Essential | no |
| Product | poly-gamma-glutamate synthetase |
| Function | capsule synthesis |
| MW, pI | 43 kDa, 5.073 |
| Gene length, protein length | 1179 bp, 393 aa |
| Immediate neighbours | capC, rbsR |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
no synthesis of the poly-gamma-glutamate capsule PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: synthesis of poly-gamma-glutamate from L-Glu PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: P96736
- KEGG entry: BSU35900
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism: activated by DegU-P
- Additional information: The operon is not expressed in the lab strain 168 due to lack of expression of DegQ resulting in inactivity of DegS and DegU
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
