Difference between revisions of "GlpF"

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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P18156 P18156]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P18156 P18156]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU09280]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU09280 BSU09280]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 22:59, 13 May 2009

  • Description: glycerol facilitator

Gene name glpF
Synonyms
Essential no
Product glycerol facilitator
Function glycerol uptake
MW, pI 28 kDa, 9.506
Gene length, protein length 822 bp, 274 aa
Immediate neighbours glpP, glpK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GlpF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: View classification (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by glucose (8-fold) (CcpA) PubMed, carbon catabolite repression, induction by glycerol
  • Regulatory mechanism: CcpA: transcription repression, catabolite repression: repression by CcpA, induction: GlpP-dependent RNA switch (transcriptional antitermination)
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
  2. Beijer L, Nilsson RP, Holmberg C, Rutberg L: The glpP and glpF genes of the glycerol regulon in Bacillus subtilis. J Gen Microbiol 1993;139:349-359. PubMed
  3. Darbon E, Servant P, Poncet S, Deutscher J (2002) Antitermination by GlpP, catabolite repression via CcpA and inducer exclusion triggered by P~GlpK dephosphorylation control Bacillus subtilis glpFK expression. Mol Microbiol 43:1039-1052. PubMed