Difference between revisions of "GyrB"

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* '''Description:''' DNA-Gyrase (subunit B) <br/><br/>
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* '''Description:''' DNA-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>Gyrase (subunit B) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|-
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|-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|''gyrB''
 
|''gyrB''
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|-
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|-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''novA ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''novA ''
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|-
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|-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]  
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|-
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|-  
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|style="background:#ABCDEF;" align="center"| '''Product''' || DNA-Gyrase (subunit B)
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>
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|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || DNA-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>Gyrase (subunit B)
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|-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>
 
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA superrcoiling, initation of replication cycle and DNA elongation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA superrcoiling, initation of replication cycle and DNA elongation
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|-
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|-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 71 kDa, 5.378   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 71 kDa, 5.378   
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|-
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|-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1914 bp, 638 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1914 bp, 638 aa  
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|-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yaaB]]'', ''[[gyrA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yaaB]]'', ''[[gyrA]]''
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|-  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/gyrB_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/gyrB_protein.txt Protein sequence]'''
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-  
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|-
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>goettingen.de/gyrB_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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|colspan="2" | '''Genetic context''' <br/> [[Image:gyrB_context.gif]]
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-  
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|-
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>goettingen.de/gyrB_protein.txt Protein sequence]'''
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|-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>
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|colspan="2" | '''Genetic context''' <br/> [[Image:gyrB_-
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>|-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>
 
|}
 
|}
  
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=== Database entries ===
 
=== Database entries ===
  
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yaaA-recF-yaaB-gyrB.html]
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yaaA-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>recF-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>yaaB-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>gyrB.html]
  
 
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10070]
 
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10070]
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
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* '''Interactions:''' [[GyrA]]-GyrB
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* '''Interactions:''' [[GyrA]]-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>GyrB
  
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* '''Localization:''' nucleoid-associated in actively growing cells [http://www.ncbi.nlm.nih.gov/sites/entrez/9539793 PubMed]
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* '''Localization:''' nucleoid-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>associated in actively growing cells [http://www.ncbi.nlm.nih.gov/sites/entrez/9539793 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00060]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>bin/www_bget?bsu+BSU00060]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:''' ''[[yaaA]]-[[recF]]-[[yaaB]]-[[gyrB]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]
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* '''Operon:''' ''[[yaaA]]-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>[[recF]]-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>[[yaaB]]-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>[[gyrB]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]
  
 
* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]
 
* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
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* '''Additional information:''' GyrB is subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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* '''Additional information:''' GyrB is subject to Clp-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
  
 
=Biological materials =
 
=Biological materials =
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
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* '''two-hybrid system:'''  
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* '''two-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>hybrid system:'''  
  
 
* '''Antibody:'''
 
* '''Antibody:'''
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=References=
 
=References=
  
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# Huang et al. (1998) Bipolar localization of Bacillus subtilis topoisomerase IV, an enzyme required for chromosome segregation. ''Proc. Natl. Acad. Sci. USA'' '''95:''' 4652-4657. [http://www.ncbi.nlm.nih.gov/sites/entrez/9539793 PubMed]
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# Huang et al. (1998) Bipolar localization of Bacillus subtilis topoisomerase IV, an enzyme required for chromosome segregation. ''Proc. Natl. Acad. Sci. USA'' '''95:''' 4652-  
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# Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>4657. [http://www.ncbi.nlm.nih.gov/sites/entrez/9539793 PubMed]
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# Ogasawara et al. (1985) Structure and function of the region of the replication origin of the ''Bacillus subtilis'' chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.''Nucl. Acids Res.'' '''11:''' 2267-2279. [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]
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# Gerth et al. (2008) Clp-  
 +
<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>dependent proteolysis down-  
 +
<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>regulates central metabolic pathways in glucose-  
 +
<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>starved Bacillus subtilis. J Bacteriol 190:321-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>331 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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# Ogasawara et al. (1985) Structure and function of the region of the replication origin of the ''Bacillus subtilis'' chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.''Nucl. Acids Res.'' '''11:''' 2267-  
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<div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>2279. [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]

Revision as of 15:01, 20 March 2009

  • Description: DNA-
the origin of the image is SubtiListGyrase (subunit B)

the origin of the image is SubtiList
the origin of the image is SubtiList
the origin of the image is SubtiList
the origin of the image is SubtiList
the origin of the image is SubtiList
the origin of the image is SubtiList
the origin of the image is SubtiList
the origin of the image is SubtiList
the origin of the image is SubtiList
the origin of the image is SubtiList
Gene name gyrB
Synonyms novA
Essential yes PubMed
Product DNA-
the origin of the image is SubtiListGyrase (subunit B)
Function DNA superrcoiling, initation of replication cycle and DNA elongation
MW, pI 71 kDa, 5.378
Gene length, protein length 1914 bp, 638 aa
Immediate neighbours yaaB, gyrA
[http://subtiwiki.uni-
the origin of the image is SubtiListgoettingen.de/gyrB_nucleotide.txt Gene sequence (+200bp) ]
[http://subtiwiki.uni-
the origin of the image is SubtiListgoettingen.de/gyrB_protein.txt Protein sequence]
Genetic context
[[Image:gyrB_-
the origin of the image is SubtiList|-
the origin of the image is SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

the origin of the image is SubtiListrecF-
the origin of the image is SubtiListyaaB-
the origin of the image is SubtiListgyrB.html]
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): ParE

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: GyrA-
the origin of the image is SubtiListGyrB
  • Localization: nucleoid-
the origin of the image is SubtiListassociated in actively growing cells PubMed

Database entries

  • Structure:
  • Swiss prot entry:
the origin of the image is SubtiListbin/www_bget?bsu+BSU00060]
  • E.C. number:

Additional information

Expression and regulation

the origin of the image is SubtiListrecF-
the origin of the image is SubtiListyaaB-
the origin of the image is SubtiListgyrB PubMed
  • Regulation:
  • Regulatory mechanism:
  • Additional information: GyrB is subject to Clp-
the origin of the image is SubtiListdependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-
the origin of the image is SubtiListhybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Huang et al. (1998) Bipolar localization of Bacillus subtilis topoisomerase IV, an enzyme required for chromosome segregation. Proc. Natl. Acad. Sci. USA 95: 4652-
the origin of the image is SubtiList4657. PubMed
  1. Gerth et al. (2008) Clp-
the origin of the image is SubtiListdependent proteolysis down-
the origin of the image is SubtiListregulates central metabolic pathways in glucose-
the origin of the image is SubtiListstarved Bacillus subtilis. J Bacteriol 190:321-
the origin of the image is SubtiList331 PubMed
  1. Ogasawara et al. (1985) Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.Nucl. Acids Res. 11: 2267-
the origin of the image is SubtiList2279. PubMed