Difference between revisions of "PAC6"
| Line 14: | Line 14: | ||
similar vectors: [[pAC5]], [[pAC7]] | similar vectors: [[pAC5]], [[pAC7]] | ||
| + | |||
| + | sequencing primer: | ||
| + | *'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’ | ||
| + | *'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘ | ||
| + | *'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’ | ||
| + | |||
Stülke J, Martin-Verstraete I, Zagorec M (1997) Induction of the ''Bacillus subtilis ptsGHI'' operon by glucose is controlled by a novel antiterminator, ''GlcT Mol Microbiol.'' '''25:''' 65-78. [http://www.ncbi.nlm.nih.gov/sites/entrez/11902727 PubMed] | Stülke J, Martin-Verstraete I, Zagorec M (1997) Induction of the ''Bacillus subtilis ptsGHI'' operon by glucose is controlled by a novel antiterminator, ''GlcT Mol Microbiol.'' '''25:''' 65-78. [http://www.ncbi.nlm.nih.gov/sites/entrez/11902727 PubMed] | ||
Revision as of 11:58, 18 March 2009
pAC6
vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: chloramphenocol resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
Stülke J, Martin-Verstraete I, Zagorec M (1997) Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT Mol Microbiol. 25: 65-78. PubMed
