Difference between revisions of "PHT08"
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The pHT08 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular production of recombinant His tagged protein with B. subtilis. The tag can be used for detection or purification of the produced protein of interest. | The pHT08 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular production of recombinant His tagged protein with B. subtilis. The tag can be used for detection or purification of the produced protein of interest. | ||
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* ''E. coli / B. subtilis'' shuttle vector | * ''E. coli / B. subtilis'' shuttle vector | ||
| − | pHT08 is a variant of [[PHT01|pHT01]], both vectors are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)] | + | pHT08 is a variant of [[PHT01|pHT01]], both vectors are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)][[File:Mobiteclogo.png|100px]] |
== References == | == References == | ||
<pubmed>17624574, 16005967, 16125412</pubmed> | <pubmed>17624574, 16005967, 16125412</pubmed> | ||
Latest revision as of 11:35, 28 August 2017
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The pHT08 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular production of recombinant His tagged protein with B. subtilis. The tag can be used for detection or purification of the produced protein of interest. The expression is based on the strong σA-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT08 is an E. coli/ B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
Features
- High level production of recombinant proteins
- Efficiently controllable gene expression (IPTG inducible)
- N-terminal 8xHis tag
- E. coli / B. subtilis shuttle vector
pHT08 is a variant of pHT01, both vectors are available from MoBiTec GmbH (Göttingen)
References
Hoang Duc Nguyen, Trang Thi Phuong Phan, Wolfgang Schumann
Expression vectors for the rapid purification of recombinant proteins in Bacillus subtilis.
Curr Microbiol: 2007, 55(2);89-93
[PubMed:17624574]
[WorldCat.org]
[DOI]
(P p)
Trang Thi Phuong Phan, Hoang Duc Nguyen, Wolfgang Schumann
Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis.
Protein Expr Purif: 2006, 46(2);189-95
[PubMed:16125412]
[WorldCat.org]
[DOI]
(P p)
Hoang Duc Nguyen, Quynh Anh Nguyen, Rita C Ferreira, Luis C S Ferreira, Linh Thuoc Tran, Wolfgang Schumann
Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability.
Plasmid: 2005, 54(3);241-8
[PubMed:16005967]
[WorldCat.org]
[DOI]
(P p)
