Difference between revisions of "PHT01"
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[[File:PHT01.PNG|600px]] | [[File:PHT01.PNG|600px]] | ||
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| − | The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the | + | The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the ''[[groES]]-[[groEL]]'' operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis. |
=== Features === | === Features === | ||
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* ''E. coli / B. subtilis'' shuttle vector | * ''E. coli / B. subtilis'' shuttle vector | ||
| − | pHT01 and further pHT-vector variants (e.g., with His, Strep or Myc tag) are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)] | + | pHT01 and further pHT-vector variants (e.g., with His, Strep or Myc tag) are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)][[File:Mobiteclogo.png|100px]] |
== References == | == References == | ||
<pubmed>17624574, 16005967, 16125412</pubmed> | <pubmed>17624574, 16005967, 16125412</pubmed> | ||
Latest revision as of 11:34, 28 August 2017
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The pHT01 is a high-level intracellular expression vector for recombinant protein production with B. subtilis. The vector is based on the strong σA-dependent promoter preceding the groES-groEL operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an E. coli/ B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
Features
- Efficiently controllable gene expression (IPTG inducible)
- High level production of recombinant proteins
- E. coli / B. subtilis shuttle vector
pHT01 and further pHT-vector variants (e.g., with His, Strep or Myc tag) are available from MoBiTec GmbH (Göttingen)
References
