Difference between revisions of "PHT01"
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| − | + | [[File:PHT01.PNG|600px]] | |
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| − | < | + | The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the ''[[groES]]-[[groEL]]'' operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis. |
| + | |||
| + | === Features === | ||
* Efficiently controllable gene expression (IPTG inducible) | * Efficiently controllable gene expression (IPTG inducible) | ||
* High level production of recombinant proteins | * High level production of recombinant proteins | ||
* ''E. coli / B. subtilis'' shuttle vector | * ''E. coli / B. subtilis'' shuttle vector | ||
| + | |||
| + | pHT01 and further pHT-vector variants (e.g., with His, Strep or Myc tag) are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)][[File:Mobiteclogo.png|100px]] | ||
| + | |||
| + | == References == | ||
| + | <pubmed>17624574, 16005967, 16125412</pubmed> | ||
Latest revision as of 11:34, 28 August 2017
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The pHT01 is a high-level intracellular expression vector for recombinant protein production with B. subtilis. The vector is based on the strong σA-dependent promoter preceding the groES-groEL operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an E. coli/ B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
Features
- Efficiently controllable gene expression (IPTG inducible)
- High level production of recombinant proteins
- E. coli / B. subtilis shuttle vector
pHT01 and further pHT-vector variants (e.g., with His, Strep or Myc tag) are available from MoBiTec GmbH (Göttingen)
References
Hoang Duc Nguyen, Trang Thi Phuong Phan, Wolfgang Schumann
Expression vectors for the rapid purification of recombinant proteins in Bacillus subtilis.
Curr Microbiol: 2007, 55(2);89-93
[PubMed:17624574]
[WorldCat.org]
[DOI]
(P p)
Trang Thi Phuong Phan, Hoang Duc Nguyen, Wolfgang Schumann
Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis.
Protein Expr Purif: 2006, 46(2);189-95
[PubMed:16125412]
[WorldCat.org]
[DOI]
(P p)
Hoang Duc Nguyen, Quynh Anh Nguyen, Rita C Ferreira, Luis C S Ferreira, Linh Thuoc Tran, Wolfgang Schumann
Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability.
Plasmid: 2005, 54(3);241-8
[PubMed:16005967]
[WorldCat.org]
[DOI]
(P p)
