gidB

168

7-methylguanosine methyltransferase specific for the 16S rRNA, glucose-inhibited division protein

Locus: BSU_41000

Molecular weight: 26.80 kDa

Isoelectric point: 6.6

Protein length: 239 aa

Gene length: 720 bp

Function: rRNA modification

Product: 7-methylguanosine methyltransferase specific for the 16S rRNA

Essential: no

Synonyms: gidB, rsmG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0357 (Galperin et al., 2021)

Gene

Coordinates: 4,208,870 → 4,209,589

Phenotypes of a mutant

• low-level resistance to streptomycin, increased level of streptomycin-resistant rpsL mutants (200 times higher than in the wild type) PubMed

The protein

Catalyzed reaction/ biological activity

• methylates the N7 position of nucleotide G535 in 16S rRNA PubMed

Protein family

• Methyltransferase superfamily (according to UniProt)

Structure

• 1XDZ (PDB)
• P25813 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: thdF-gidA-gidB-noc

  Description: PubMed

  Regulation

  • constitutively expressed PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon
  • DnaA: repression, PubMed, in dnaA regulon

Biological materials

Mutant

• MGNA-B875 (gidB::erm), available at the NBRP B. subtilis, Japan
• BKE41000 (ΔgidB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCAATATTCATGCCGTCAT,  downstream forward: _UP4_TAATTTAGACAGCCTGATAT
• BKK41000 (ΔgidB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCAATATTCATGCCGTCAT,  downstream forward: _UP4_TAATTTAGACAGCCTGATAT

References

Loading