hbuT

168

sporulation protein, transporter for 3-hydroxybutyrate

Locus: BSU_39000

Molecular weight: 49.98 kDa

Isoelectric point: 8.9

Protein length: 472 aa

Gene length: 1419 bp

Function: 3-hydroxybutyrate utilization

Product: transporter for 3-hydroxybutyrate

Essential: no

Synonyms: hbuT, yxjC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2610 (Galperin et al., 2021)

Gene

Coordinates: 4,002,716 → 4,004,134

Phenotypes of a mutant

• reduced uptake of 3-hydroxybutyrate during sporulation PubMed

The protein

Catalyzed reaction/ biological activity

• uptake of 3-hydroxybutyrate PubMed

Protein family

• CitM transporter family (TC 2.A.11) (according to UniProt)

Structure

• P42314 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: hbuT-scoA-scoB-yxjF

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-B727 (yxjC::erm), available at the NBRP B. subtilis, Japan
• BKE39000 (ΔhbuT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACACACACATCCCCCGTTT,  downstream forward: _UP4_TAGAAGAAAACGAAAGGGGC
• BKK39000 (ΔhbuT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACACACACATCCCCCGTTT,  downstream forward: _UP4_TAGAAGAAAACGAAAGGGGC

References

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